Next Generation Sequencing « Erasmus Center for Biomics

Next Generation Sequencing Platforms
	Biomics operates a fexible NGS pipeline with HiSeq2500, HiSeq4000 and Miseq sequencers. Please check our overview comparing the sequencers. The Illumina NGS platforms are superior due to the large number of short sequence reads and the speed of production

Omni-ATAC sequencing		ChIPThruplex V2 Sequencing
(from: Buenrostro et al)	Assay for Transposase-Accessible Chromatin with high throughput sequencing	(from: Sleutels et al)	Multiplexed Chromatin Immuno Precipitation Sequencing e.g. histone modifications and variants, transcription factors, chromatin modifyers

CRISPR Sequencing		Chromatin Conformation Sequencing
	For CRISPR sequencing the customer performs a CRISPR Cas9 screen in which guide RNAs are built into the Sample DNA	(from: Soler et al)	Chromatin Conformation Capture (3C) Chromatin Interactome Chromatome

Exome Sequencing		Genome Sequencing
	Whole Exome Capture Sequencing, e.g. Agilent SureSelect Clinical Research ExomeCustom Exome Capture Sequencing e.g. Agilent SureSelectVariant Calling, Annotation & Analyses		(De Novo) genome sequencing e.g. bacteria, viruses, animal models Genome Assembly, Annotation, Comparsion

Long read sequencing ^NEW!		RNA Sequencing
	MinION sequencing technology from Oxford Nanopore^NEW!		RNA sequencing, Transcriptome, Alternative Splicing gene activity, Expression analyses

Custom Sequencing Projects
	For custom sequencing projects, modifications of sequencing applications development of new sequencing strategies, Please contact us


For custom sequencing projects. Please contact us.